Further analysis of selected genes from the RNAi screen cluster #1, by Ca²⁺ imaging (A, B) and by electrophysiology (C-G).

Abstract

<p>The genomic mutants <i>C27B7</i>.<i>7(ok2978)</i>, <i>inx-10(ok2714)</i>, <i>inx-8(gk42)</i>, <i>spp-10(gk349)</i> and <i>erp-1(ok462)</i> were crossed either to the ChR2(C128S); RCaMP strain for Ca²⁺ imaging, or to the <i>zxIs6</i> ChR2(H134R) strain, for measuring photo-ePSCs. In A and B, normalized Ca²⁺ signal difference traces are shown as color coded, maximum normalized data, based on the mean ΔF/F₀ Ca²⁺ response (± SEM) from bulk measurements of RNAi or mutant and control (n = 3–5 experiments, ~1000 animals each). In E—G, the averaged photo-ePSC measurements of the respective wild type animals (black), <i>C27B7</i>.<i>7(ok2978)</i> (red), <i>inx-10(ok2714)</i> (yellow), <i>inx-8(gk42)</i> (green), <i>spp-10(gk349)</i> (blue), or <i>erp-1(ok462)</i> mutants (orange) are shown as inward currents in pA ± SEM (left panels). Statistically significant differences were calculated by one-way ANOVA for individual time points, always compared to wild type (* P < 0.05), or for the whole data set as two-way ANOVA (** P < 0.01, **P<0.001). Animals were stimulated for 10 ms with blue light at a frequency of 0.5 Hz (n = 7–12). On the right, the same data are represented, but normalized to the mean peak currents of the first stimulus. For representative original current records, as well as for mini ePSC current and frequency analysis, see <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135584#pone.0135584.s005" target="_blank">S5 Fig</a>.</b></p