Ratiometric Signaling of Hypochlorite by the Oxidative
Cleavage of Sulfonhydrazide-Based Rhodamine–Dansyl Dyad
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Abstract
A reaction-based
probe <b>1</b> for hypochlorite signaling was designed by the
conjugation of two fluorophores, rhodamine and dansyl moieties, by
the reaction of rhodamine B base with dansylhydrazine. Probe <b>1</b> exhibited pronounced hypochlorite-selective chromogenic
and fluorescent signaling behavior over other oxidants used in practical
applications, such as hydrogen peroxide, peracetic acid, and ammonium
persulfate, as well as commonly encountered metal ions and anions.
Signaling was attributed to the hypochlorite-induced oxidative cleavage
of the sulfonhydrazide linkage of the probe. In particular, favorable
ratiometric fluorescence signaling was possible by utilizing the emissions
of the two fluorophores. A detection limit of 1.13 × 10<sup>–6</sup> M (0.058 ppm) was estimated for the determination of hypochlorite.
A paper-based test strip was prepared and was used as a semiquantitative
indicator for the presence of hypochlorite in aqueous solutions. The
probe was also successfully applied for the determination of hypochlorite
in practical tap water samples