<i>Pm</i>–mediated basal expression is low and compares favorably to <i>Ptet-</i>mediated basal expression in <i>Mtb</i>.

Abstract

<p>(A-B) <i>Mtb</i> transformed with pMDX-luc or pUV15tetORm::luciferase was diluted to OD<sub>600</sub> 0.005 in the presence or absence of m-toluate (1.5 mM) or atc (200 ng/ml), respectively. Samples were grown in triplicates and monitored for 7 days registering OD<sub>600</sub> at 2, 4 and 7 days. Basal expression from <i>Pm</i> and <i>Ptet</i> is presented by level of luciferase produced by <i>Mtb</i> pMDX-luc and <i>Mtb</i> pUV15tetORm::luciferase. (A) shows the luciferase expression over time in induced sample and (B) shows the basal expression from uninduced samples over time. The results represent two independent experiments.</p

    Similar works

    Full text

    thumbnail-image

    Available Versions