Quencher Group Induced High Specificity Detection
of Telomerase in Clear and Bloody Urines by AIEgens
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Abstract
Telomerase is a widely used tumor
biomarker for early cancer diagnosis.
On the basis of the combined use of aggregation-induced emission (AIE)
fluorogens and quencher, a quencher group induced high specificity
strategy for detection of telomerase activity from cell extracts and
cancer patients’ urine specimens was creatively developed.
In the absence of telomerase, fluorescence background is extremely
low due to the short distance between quencher and AIE dye. In the
addition of telomerase, fluorescence enhances significantly. The telomerase
activity in the E-J, MCF-7, and HeLa extracts equivalent to 5–10 000
cells can be detected by this method in ∼1 h. Furthermore,
the distinguishing of telomerase extracted from 38 cancer and 15 normal
urine specimens confirms the reliability and practicality of this
protocol. In contrast to our previous results (<i>Anal. Chem.</i> <b>2015</b>, <i>87</i>, 6822–6827), these
advanced experiments obtain more remarkable specificity