Additional file 2: Figure S1. of ALK alteration is a frequent event in aggressive breast cancers

Abstract

Biochemical fractionation of ALK expression in breast cancer cell lines. (A) Nuclear-free cytosolic and nuclear extracts were isolated from MDA-MB231 and MCF12A cells and immunoblotted with antibodies against ALK and tubulin. (B) Relative expression of ALK was calculated using spot densitometry on cytosolic and nuclear immunoblots of MDA-MB231 and MCF12A cells. Expression of ALK was normalized with tubulin