<p><b>(A and B)</b>. Affinity chromatography of LLC-MK<sub>2</sub> cell extract using immobilized peptide TS9, eluted sequentially with 1% SDS and 8M urea, were analyzed by SDS-PAGE and Coomassie staining <b>(A)</b> or Western-blot using anti-KRT18 antibody <b>(B)</b>. In <b>(A)</b>, proteins with calculated masses of 49, 53, 59 and 66 kDa identified by Coomassie staining (arrows a-d, respectively) were excised from the gel and analyzed by mass spectrometry. <b>(B)</b> Immunoreactivity of anti-KRT18 antibody with the 1% SDS and 8M urea eluates; recombinant KRT18 was used as positive control. <b>(C)</b> Binding of phage TS9, FLY and Fd-tet (insertless phage) to BSA, recombinant human cytokeratins 8, 14, 18, 19 and 20, vimentin or gelatin. Mean ± SEM of a representative experiment performed in triplicate are shown (* indicates p<0.05, two-way ANOVA).</p
