Abstract

<p><b>(A and B)</b> Effect of peptide TS9 or the control scramble peptide (200 μM) on LLC-MK<sub>2</sub> host cell infection by <i>T</i>. <i>cruzi</i>. In <b>(A)</b> the number of infected cells is indicated. Values are expressed as relative the number of infected cells (vehicle-only treatment values were set to 1). Mean ± SEM of a representative experiment performed in triplicate are shown. In <b>(B)</b> the number of parasites per infected host cells is shown. Data are presented in box plots in which the boxes define the 25th and 75th percentiles, with a line at a median and whiskers defining the maximum and minimum values of experiments performed in triplicate (*** p<0.001, one-way ANOVA). <b>(C)</b> Representative pictures of microscope fields used for the quantification of parasite invasion. Cells were stained with DAPI. The arrows indicate infected cells with amastigotes in the cytoplasm. (<b>D</b>) Dose dependence effect of peptide TS9 on LLC-MK<sub>2</sub> host cell infection by the parasite. (<b>E</b>) Infection assay performed at 4°C to prevent parasite from entering host cells in the presence or absence of peptides TS9 or scramble (200 μM). (<b>F</b>) Effect of Tc85-11<sup>LamG</sup> protein on parasite host cell infection.</p

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