<p><b>(A)</b> Flowchart summarizing the pipeline for <i>in silico</i> selection, bacteriophage display and the <i>in vitro</i> functional assay (BRASIL method) for the identification of conserved peptides derived from the gp85/TS family with cell binding properties. <b>(B)</b> Quantification of phage binding to LLC-MK<sub>2</sub> cells using the BRASIL methodology and quantitative PCR (qPCR). Phage binding results were normalized relative to the control phage Fd-tet (insertless phage). Mean ± standard error of the means (SEM) from four samples are shown. Significant cell binding was observed for phage FLY and TS9 (* p<0.05 and *** p<0.001; one way ANOVA, N = 4). <b>(C)</b> Peptide competition assay. Effect of synthetic peptide TS9 and the control scramble peptide in phage TS9 binding to LLC-MK<sub>2</sub>. Phage binding results were normalized relative to the control phage Fd-tet. Mean ± SEM of a representative experiment performed in triplicate are shown.</p
