Stimulation of <i>eh</i>Dmc1-mediated DNA strand exchange activity by mHop2-Mnd1 and Ca²⁺.

Abstract

<p><b>A.</b> Schematic of oligonucleotide strand exchange assay. <b>B.</b> A time course analysis of <i>eh</i>Dmc1 strand exchange activity (top panel) in the presence of 2 mM calcium (Ca²⁺), mHop2-Mnd1 (H2M1), and the combination of calcium and mHop2-Mnd1 (Ca²⁺ H2M1), as indicated. At the indicated times, an aliquot was removed and deproteinized. The reaction products were separated on 12% native polyacrylamide gels, and the gels were analyzed by a phosphorimager. Lane 1 is devoid of protein (Bl.). <b>C.</b> Mean values from three individual experiments from <b>B</b> were graphed. Error bars represent SEM. <b>D.</b> A 5 min time course analysis of <i>eh</i>Dmc1 strand exchange activity in the presence of mHop2-Mnd1 (H2M1) or the combination of 2 mM calcium and mHop2-Mnd1 (Ca²⁺ H2M1), as indicated. At the indicated times, an aliquot was removed, deproteinized and processed as described in <b>B</b>. Lane 1 (Bl.) is devoid of protein. <b>E.</b> Mean values of three independent experiments from <b>D</b> were plotted. Error bars represent SEM.</p