<div><p>Methanolic extract of Golden rain leaves was fractionated by column chromatography on silica gel and 18 fractions were obtained. Antimicrobial activities of fractions were investigated against <i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> as quality control bacteria and fungus <i>Pyricularia grisea</i> which causes Blast disease in rice. Fractions showed more antibacterial activity at 0.04 g/mL concentration only on <i>B. subtilis</i> and <i>S. aureus</i> as gram positive bacteria. Also, three fractions indicated excellent antifungal effect on fungus <i>P. grisea</i>. Moreover, in the present study, fractions that showed very good effect on microorganisms were used for gas chromatography-mass spectrometry analysis to identify different phytochemicals.</p></div
