Analysis of the purified recombinant <i>Gly m 4l</i> protein.

Abstract

<p>(A) The recombinant Gly m 4l protein induced with 0.5 mM IPTG at 37°C for 1–5 h in <i>E</i>.<i>coli</i> BL21 (DE3). (B) SDS-PAGE analysis of the purified recombinant Gly m 4l protein using His-Bind Kits. (C) The purified recombinant Gly m 4l protein analyzed by immunoblotting using anti-His antibody.</p