Chaxapeptin, a Lasso
Peptide from Extremotolerant <i>Streptomyces leeuwenhoekii</i> Strain C58 from the Hyperarid
Atacama Desert
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Abstract
Lasso
peptides are ribosomally synthesized and post-translationally
modified peptides (RiPPs) that possess a unique “lariat knot”
structural motif. Genome mining-targeted discovery of new natural
products from microbes obtained from extreme environments has led
to the identification of a gene cluster directing the biosynthesis
of a new lasso peptide, designated as chaxapeptin <b>1</b>,
in the genome of <i>Streptomyces leeuwenhoekii</i> strain
C58 isolated from the Atacama Desert. Subsequently, <b>1</b> was isolated and characterized using high-resolution electrospray
ionization mass spectrometry and nuclear magnetic resonance methods.
The lasso nature of <b>1</b> was confirmed by calculating its
nuclear Overhauser effect restraint-based solution structure. Chaxapeptin <b>1</b> displayed a significant inhibitory activity in a cell invasion
assay with human lung cancer cell line A549