<div><p></p><p><i>Background</i>: Copy Number Variants (CNVs) contribute to a large fraction of genetic diversity and some of them have been reported to offer an evolutionary advantage.</p><p><i>Aim</i>: To identify CNVs in pigmentary loci that could contribute to human skin pigmentation diversity.</p><p><i>Subjects and methods</i>: This study assessed the existence of CNVs in every exon of candidate genes: <i>TYR, TYRP1, DCT, MC1R</i> and <i>SLC24A5</i>, using the Multiplex Amplifiable Probe Hybridization technique (MAPH). This study analysed a total of 99 DNA samples of unrelated individuals from different populations. Validation and further analysis in a larger Spanish sample were performed by RT-qPCR.</p><p><i>Results</i>: Five CNVs were identified by MAPH: <i>DCT</i> exons 4 and 8, <i>TYR</i> exon 1 and <i>SLC24A5</i> exons 1 and 4. Real-time quantitative PCR (RT-qPCR) confirmed the CNV in exon 1 of <i>SLC24A5</i>. This study further analysed the 5′ promoter region of <i>SLC24A5</i> and found another CNV in this region. However, no association was found between the CNV and the degree of pigmentation.</p><p><i>Conclusion</i>: Although the functional role of these structural variants in pigmentation should be the subject of future work, the results emphasize the need to consider all classes of variation (both SNPs and CNVs) when exploring the genetics of skin pigmentation.</p></div