Affymetrix QuantiGene 2.0 custom panel 21522 for analysis of macrophage polarization. Figure S1. Genotype validation of splenocyte populations. Figure S2. Genotype validation of BMDMs. Figure S3. TLR9 in vivo activation induces upregulation of serum cytokines and chemokines at similar levels in Casp8 fl/fl and Cre LysM Casp8 fl/fl mice. Figure S4. Caspase-8âdeficient splenic myeloid populations are not predisposed to aberrant death. Figure S5. Caspase-8âdeficient BMDMs express Fas. Figure S6. Caspase-8âdeficient BMDMs undergo caspase-independent cell death in response to apoptotic stimuli. Figure S7. Caspase-8 deficiency in macrophages alters the response to TLR activation in vitro. Figure S8. Caspase-8 deficiency in macrophages alters the genetic profile in response to macrophage polarization in vitro. (PDF 5409 kb