CRVP379 antisera blocks infection of DENV in mosquitoes.

Abstract

<p>A/B. CRVP379 antisera inhibits DENV infection in mosquito cells. Aag2 (A) or Huh7 (B) cells were either incubated with antisera against CRVP379 or control preimmune sera for 2h at RT and then infected with DENV (pretreatment group) or antisera against CRVP379 or control preimmune sera was incubated with DENV for 1h at RT and then added to cells (simultaneous group). Infection was analyzed by qRT-PCR at 24 hpi. C/D. CRVP379 antisera inhibits DENV infection in mosquitoes. <i>Ae</i>. <i>aegypti</i> were fed a mixture of blood, DENV and either CRVP379 antisera or preimmune sera as indicated. Antisera were used at dilutions of 1/10 or 1/100 (C). Infection rates in midguts with CRVP379 antisera ranged from to .00008785–.07833 ng DENV E/ng actin. A separate group of mosquitoes was fed antisera against control mosquito proteins, MMP and PC (D). At 3 dpi, mosquito MG were dissected and qRT-PCR analysis done to quantify DENV infection. Results are shown as ng DENV E normalized to mosquito actin. Each data point represents one MG.</p