<p><b>A)</b> Structure of E140Q Bd-NDPSase bound to ADPR (PDB ID 5C7T). <b>B)</b> Sequence alignment of the Ec-ADPRase (PDB ID 1KHZ) and Ec-NDPSase (PDB ID 3O61) to Bd-NDPSase. The N-terminal domain (residues 1–44) consists of an antiparallel beta sheet (β1- β3) and is denoted by green. The Nudix fold consists of a mixed beta sheet (β1- β10, yellow) flanked by helix α1 (cyan) on one side and helices α2 and α3 (residues 148–182, gray) on the other side. The location of the E140Q mutation on loop L9 (magenta) is denoted by an asterisk. The prime symbol (‘) denotes residues of the opposite monomer (lighter color shade).</p
