Spherical
Nanoparticle Supported Lipid Bilayers for
the Structural Study of Membrane Geometry-Sensitive Molecules
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Abstract
Many
essential cellular processes including endocytosis and vesicle
trafficking require alteration of membrane geometry. These changes
are usually mediated by proteins that can sense and/or induce membrane
curvature. Using spherical nanoparticle supported lipid bilayers (SSLBs),
we characterize how SpoVM, a bacterial development factor, interacts
with differently curved membranes by magic angle spinning solid-state
NMR. Our results demonstrate that SSLBs are an effective system for
structural and topological studies of membrane geometry-sensitive
molecules