<p>CD1d dimers were incubated with 40x molar excess of indicated glycolipid in presence of 0.05% of indicated surfactant or without surfactant o.n. at 37°C. After <i>in vitro</i> loading, the indicated amount of CD1d dimers was used for staining of 10<sup>5</sup> iNKT TCR transduced cells, dimer binding was revealed by staining with PE labeled donkey F(ab′)<sub>2</sub> fragment anti-mouse IgG (H+L). Binding presented as relative staining, i.e. ratio between geometric means of CD1d dimer and CD3 antibody staining was performed simultaneously. In total, staining was performed three times independently using oligomers derived from two independent loadings. Hierarchy of surfactant efficacy between experiments is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143449#pone.0143449.s001" target="_blank">S1 Fig</a>. Note that scales differ in order to facilitate visualization different hierarchies of binding caused by the different surfactants.</p
