Additional file 5: Showing Notch-1, NICD, HES-1, Nestin, TNF-α, IL-1β and iNOS expression (red) in GFAP (green) reactive astrocytes at 21 d after MCAO (M) and after scutellarin treatment (M + S). Note that the expression is diminished in MCAO but remained more intense with scutellarin treatment. Scale bars: 50 µm. DAPI-blue