Additional file 2: Scutellarin enhanced IL-1β (A1, A2, A3) and iNOS (B1, B2, B3) expression in TNC1 via BV-2-conditioned medium. In TNC1 astrocytes treated with CM, moderate expression of IL-1β and iNOS was detected (A1, B1). The expression was noticeably increased in CM + L (A2, B2) and further enhanced upon incubation with CM + SL for 24 h (A3, B3) with long cytoplasmic processes with expansions projected by TNC1 astrocytes (A3, B3). Scale bars: 20 μm