<p>100μg of testicular- or caudal- sperm protein was incubated for 2h without or with 300U of λ-PP at 30°C (<b>A</b>), or without or with 32U of CIP at 37°C (<b>B</b>) followed by NIA using 20ng of protein lysate thus treated to detect the GRP78 peaks. Figure shows representative isoelectropherograms for testicular- (Upper panel) and caudal- sperm (Lower panel). Figures in inset represent bar diagrams of the cumulative data. Values are mean ± Standard Deviation (SD). Statistical significance was determined using Paired Students’ ‘t’ test and significance level set at P ≤ 0.05.</p
