Interfacial Structure and Transformation of Guanine-Rich
Oligonucleotides on Solid Supported Lipid Bilayer Investigated by
Sum Frequency Generation Vibrational Spectroscopy
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Abstract
Lipid
membrane-anchored guanine-rich oligonucleotides with non-Watson–Crick
structures can perform structure transformation in a controllable
and reversible manner upon the external stimuli. Elucidating the mechanisms
of their interaction and transformation is the key to understand medical
applicability and functioning feasibility of these oligonucleotides.
In this study, the molecular structure and interfacial transformation
kinetics of guanine-rich oligonucleotides at model cell membrane were
investigated by sum frequency generation vibrational spectroscopy
(SFG-VS) in real time and <i>in situ</i>. The conformations
of oligonucleotides are obtained by analyzing the SFG spectra in the
“fingerprint” region. The results indicate that the
electrostatic interaction and hydrophobic interaction are both important
to the interfacial adsorption and transformation of oligonucleotides.
The tilt angles of oligonucleotides with different conformations were
also calculated. Molecular insights into interfacial oligonucleotides
will help researchers to control the oligonucleotide–lipid
membrane interactions in a desired manner and improve the reproductivity,
stability, and reversibility of oligonucleotide-based applications