Structure and Biosynthesis of the Antibiotic Bottromycin D

Abstract

Drug resistant infectious diseases are quickly becoming a global health crisis. While <i>Streptomyces</i> spp. have been a major source of antibiotics over the past 50 years, efficient methods are needed to identify new antibiotics and greatly improve the rate of discovery. LCMS-based metabolomics were applied to analyze extracts of 50 <i>Streptomyes</i> spp. Using this methodology, we discovered bottromycin D and used whole genome sequencing to determine its biosynthesis by a ribosomal pathway