Influence
of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric
Carbonyl Reductions by Engineered Escherichia coli
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Abstract
This
study was designed to determine whether whole cells or crude enzyme
extracts are more effective for preparative-scale ketone reductions
by dehydrogenases as well as learning which cofactor regeneration
scheme is most effective. Based on results from three representative
ketone substrates (an α-fluoro-β-keto ester, a <i>bis</i>-trifluoromethylated acetophenone, and a symmetrical
β-diketone), our results demonstrate that several nicotinamide
cofactor regeneration strategies can be applied to preparative-scale
dehydrogenase-catalyzed reactions successfully