Globally Visualizing the Microtubule-Dependent Transport
Behaviors of Influenza Virus in Live Cells
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Abstract
Understanding
the microtubule-dependent behaviors of viruses in
live cells is very meaningful for revealing the mechanisms of virus
infection and endocytosis. Herein, we used a quantum dots-based single-particle
tracking technique to dynamically and globally visualize the microtubule-dependent
transport behaviors of influenza virus in live cells. We found that
the intersection configuration of microtubules can interfere with
the transport behaviors of the virus in live cells, which lead to
the changing and long-time pausing of the transport behavior of viruses.
Our results revealed that most of the viruses moved along straight
microtubules rapidly and unidirectionally from the cell periphery
to the microtubule organizing center (MTOC) near the bottom of the
cell, and the viruses were confined in the grid of microtubules near
the top of the cell and at the MTOC near the bottom of the cell. These
results provided deep insights into the influence of entire microtubule
geometry on the virus infection