Direct Drug Cocktail Analyses Using Microscale Vortex-Assisted Electroporation
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Abstract
Combination therapy has become one
of the leading approaches for
treating complex diseases because it coadministers clinically proven
drugs to concurrently target multiple signaling pathways of diseased
cells. Identification of synergic drug combinations at their respective
effective doses without unwanted accumulative side effects is the
key to success for such therapy. In this work, we demonstrate the
feasibility of the vortex-assisted microfluidic electroporation system
for direct drug cocktail analyses where drug substances were individually
delivered into cytosols in a sequential and dosage-controlled manner.
Through quantitative analyses, the synergic combinational dosage ratios
of the chemotherapeutic drug and the anticancer flavonoid were identified.
When integrated with high-throughput label-free rare cell purification
techniques, the presented system has the potential for development
of personalized medicines as the system would be capable of comprehensively
assessing drug combinations directly on patients’ cellular
samples