Polymerization of Affinity Ligands on a Surface for
Enhanced Ligand Display and Cell Binding
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Abstract
Surfaces
functionalized with affinity ligands have been widely
studied for applications such as biological separations and cell regulation.
While individual ligands can be directly conjugated onto a surface,
it is often important to conjugate polyvalent ligands onto the surface
to enhance ligand display. This study was aimed at exploring a method
for surface functionalization via polymerization of affinity ligands,
which was achieved through ligand hybridization with DNA polymers
protruding from the surface. The surface with polyvalent ligands was
evaluated via aptamer-mediated cell binding. The results show that
this surface bound target cells more effectively than a surface directly
functionalized with individual ligands in situations with either equal
amounts of ligand display or equal amounts of surface reaction sites.
Therefore, this study has demonstrated a new strategy for surface
functionalization to enhance ligand display and cell binding. This
strategy may find broad applications in settings where surface area
is limited or the surface of a material does not possess sufficient
reaction sites