Metabolomic and Lipidomic
Analysis of Serum from Mice
Exposed to an Internal Emitter, Cesium-137, Using a Shotgun LC–MS<sup>E</sup> Approach
- Publication date
- Publisher
Abstract
In
this study ultra performance liquid chromatography (UPLC) coupled
to time-of-flight mass spectrometry in the MS<sup>E</sup> mode was
used for rapid and comprehensive analysis of metabolites in the serum
of mice exposed to internal exposure by Cesium-137 (<sup>137</sup>Cs). The effects of exposure to <sup>137</sup>Cs were studied at
several time points after injection of <sup>137</sup>CsCl in mice.
Over 1800 spectral features were detected in the serum of mice in
positive and negative electrospray ionization modes combined. Detailed
statistical analysis revealed that several metabolites associated
with amino acid metabolism, fatty acid metabolism, and the TCA cycle
were significantly perturbed in the serum of <sup>137</sup>Cs-exposed
mice compared with that of control mice. While metabolites associated
with the TCA cycle and glycolysis increased in their serum abundances,
fatty acids such as linoleic acid and palmitic acid were detected
at lower levels in serum after <sup>137</sup>Cs exposure. Furthermore,
phosphatidylcholines (PCs) were among the most perturbed ions in the
serum of <sup>137</sup>Cs-exposed mice. This is the first study on
the effects of exposure by an internal emitter in serum using a UPLC–MS<sup>E</sup> approach. The results have put forth a panel of metabolites,
which may serve as potential serum markers to <sup>137</sup>Cs exposure