3D Printed Microfluidic Device with Integrated Biosensors for Online Analysis
of Subcutaneous Human Microdialysate
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Abstract
This
work presents the design, fabrication, and characterization
of a robust 3D printed microfluidic analysis system that integrates
with FDA-approved clinical microdialysis probes for continuous monitoring
of human tissue metabolite levels. The microfluidic device incorporates
removable needle type integrated biosensors for glucose and lactate,
which are optimized for high tissue concentrations, housed in novel
3D printed electrode holders. A soft compressible 3D printed elastomer
at the base of the holder ensures a good seal with the microfluidic
chip. Optimization of the channel size significantly improves the
response time of the sensor. As a proof-of-concept study, our microfluidic
device was coupled to lab-built wireless potentiostats and used to
monitor real-time subcutaneous glucose and lactate levels in cyclists
undergoing a training regime