<i>In Vitro</i> Selection for Small-Molecule-Triggered
Strand Displacement and Riboswitch Activity
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Abstract
An <i>in vitro</i> selection method for ligand-responsive
RNA sensors was developed that exploited strand displacement reactions.
The RNA library was based on the thiamine pyrophosphate (TPP) riboswitch,
and RNA sequences capable of hybridizing to a target duplex DNA in
a TPP regulated manner were identified. After three rounds of selection,
RNA molecules that mediated a strand exchange reaction upon TPP binding
were enriched. The enriched sequences also showed riboswitch activity.
Our results demonstrated that small-molecule-responsive nucleic acid
sensors can be selected to control the activity of target nucleic
acid circuitry