Additional file 1: Figure S1. Secreted protein by transformant of PC-3-7 and QM9414. A: Avicel cultivation; 10 µL of the supernatant from day 5 of PC-3-7 series and day 6 of QM9414 series were subjected to SDS-PAGE. Gels were stained by Coomassie Brilliant Blue. B: Cellobiose cultivation; 20 µL of supernatant from day 2.5 of PC-3-7 series and day 3 of QM9414 series were subjected to SDS-PAGE. Gel of Avicel cultivation was stained by Coomassie Brilliant Blue and that of cellobiose cultivation was silver stained. Figure S2. Gene expression profile of PC-3-7 transformants on Avicel induction. Genes analyzed were cbh1 (A), egl1 (B), bgl2 (C), and xyr1 (D). Stippled gray bar represents PC-3-7, solid gray bar represents PC-Wbgl2, and the shaded gray bar indicates PC-∆bgl2. Values represent the relative expression of each gene normalized to act1 as an internal control. Values represent the means of triplicate experiments. Error bars indicate standard deviations. Figure S3. Effect of bgl2 mutation and disruption in T. reesei QM9414. A: specific activity of the intracellular cellobiase from transformants QM9414, QM-Mbgl2 and QM-∆bgl2. Cellobiase activity is derived from the mean of triplicate experiments. Error bars indicate standard deviation. B: HPLC analysis of transglycosylation products using cell-free extracts from transformants. Details are as in Fig. 2. Putative transglycosylation products are indicated by arrows. Figure S4. Southern analysis of transformants for bgl2 analysis. A: the schematic representation of genomic structure of each transformants. B: results of hybridization by gene specific probe for bgl2 and pyr4. Genomic DNA of each transformants was digested by SacI. Lane M represents molecular marker. Table S1. PCR primers used for plasmid construction. Table S2. primers used for real-time quantitative PCR
