Coelenterazine chemiluminescence is dependent upon superoxide anion concentrations <i>in vitro</i>.

Abstract

<p>(A) HeLa cells were treated with range of 0 units/mL to 300 units/mL of PEG-SOD and imaged sequentially. The image was taken at 95 minutes post PEG-SOD addition. (B) Quantification of HeLa (black) or buffer (white) coelenterazine chemiluminescence for each concentration 95 minutes post PEG-SOD addition (n = 3). (C) HeLa cells were treated with range of 0 units/mL to 600 units/mL of PEG-CAT and imaged sequentially. Image is 95 minutes post PEG-CAT addition. (D) Quantification of HeLa (black) or buffer (white) coelenterazine chemiluminescence for each concentration 95 minutes post PEG-CAT addition (n = 3). (E) HeLa cells were treated with range of 0 μmol/L to 500 μmol/L of uric acid and imaged sequentially. Image is 95 minutes post uric acid addition. (F) Quantification of HeLa (black) or buffer (white) coelenterazine chemiluminescence for each concentration 95 minutes post uric acid addition (n = 3). (Error bars represent the mean ± SEM, and * denotes significant differences of P < 0.02.)</p

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