FIG 1 Phylogenetic relationships of the E. bieneusi genotypes identified in this study and other reported genotypes, as inferred by neighbor-joining analysis of ITS gene sequences based on the distances calculated using the Kimura 2-parameter model. Bootstrap values >50Â % from 1000 replicates are shown on the nodes. The tree was rooted with GenBank sequence AF059610 from a dog. Known genotypes observed in goats and sheep are marked with open squares and triangles, and the new genotypes in goats and sheep from this study are indicated by filled squares and triangles. (TIF 2078 kb