Targeting and genotyping strategy of the Lst1 KO strain. Mice were genotyped by using different primer pairs leading to PCR products which allowed the identification of wild-type (310 bp), KO (400 bp) and heterozygous animals (310 bp and 400 bp) (A). Primers (blue arrows) used for genotyping: Lst1-fwd: 5′ - GTG CGT GCT CAG TCA CAC TA – 3′; Lst1-rev: 3′ - AGG CCA ACA ATA AGT CCT TAC – 5′; neofwd: 3′ - TCA TTC TCA GTA TTG TTT TGC C – 5′. Abbreviations: lacZ: ß-galactosidase coding sequence from the E.coli lacZ gene, hubiP: promoter from the human ubiquitin C gene, neor: coding sequence for neomycin phosphotransferase, p(A): polyadenylation signal, black arrow: direction of gene transcription, black boxes: Lst1 coding region. To confirm the knock-out of the Lst1 gene in C57BL/6 N-Lst1tm1(KOMP)Vlcg mice we performed reverse transcription. Figure S2. No difference in changes of body weight or survival rate between Lst1 KO and C57BL/6 J mice after infection with H3N2 influenza A virus Male C57BL/6 N-Lst1 tm1(KOMP)Vlcg (n = 7) and C57BL/6 J mice (n = 10) were infected intranasally with 2x10³ FFU H3N2 virus (A/HK/01/68) in 20 μl PBS. Body weight (A) and survival (B) were determined for each day p.i. for a period of 14 days. Percent weight change is shown with reference to the starting body weight. Significances were calculated using non-parametric Mann Whitney U test. (p < 0.01 for day 1) and Logrank test for survival rates (not significant). (PDF 230 kb
