Additional file 3. Modulated mRNA concentrations after stimulating pbMEC, pbMFC, RAW 264.7 or MAC-T with E. coli 1303 for various times to illustrate basic and full-scale mRNA expression in these cell types. Values are means from two biological replica experiments, each assayed in duplicate (Âą SEM) of relative copy numbers; grey underlay, significant induction; red underlay, significant down regulation, fold change > 2, P < 0.05 vs. unstimulated control. Bonferroniâs correction for multiple analyses was applied