Pearson correlation plots of RNA-Seq reads. Correlation between individual biological replicates in each time-point (5 and 7d) and treatment (control, GAc and shade) using ln-transformed read counts for the DEG as input. All correlation values are significant at p-value ≤ 0.001. (PDF 140 kb

A Aziz

A Botton

A Conesa

A Evans

A Gomez-Cadenas

A Malik

A Schaller

AG Reynolds

AK Gupta

AM Bolger

AM Evans

AP Singh

B Hed

BN Kunkel

C Bonghi

C Cheng

C Li

C Lopes

C Nunes

C Quast

C Trapnell

CE Niederhuth

CL Arias

Claudia Sánchez

Cristina M. Oliveira

D Granot

D Kim

D Lorenz

D Rumeau

DC Ferree

E Baena-González

E Giulia

E Keunen

FJ Perez

FJ Rohlf

G Galili

G Lebon

H Saika

H Zhu

HS Chung

HW Caspari

I Waduwara-Jayabahu

J Jouhet

J Krasensky

J Lastdrager

J Mahouachi

J Vandesompele

J Zhang

J-K Zhu

J-M Davière

JE Taylor

JM Alamillo

Joana Fino

José C. Ramalho

K Graeber

KA Roubelakis

L Braidwood

L Chai

L Corelli

L Giacomelli

LH Estornell

Luis F. Goulao

M Büttner

M Kanehisa

M Sakamoto

M Sawicki

M Zibordi

M-K Chen

MA Butenko

MA Else

MA Keller

MC Gomez-Jimenez

MC Parra-Lobato

MC Vasconcelos

MI Love

MJ Quiles

MK Zhiponova

ML Tucker

MM Basu

N Smirnoff

NE Looney

NK Dokoozlian

O Jaillon

Octávio S. Paulo

P Wang

R Bessis

R Byers

R Rasmussen

R Srivastava

R Suzuki

R Yuan

RL Tatusov

Roberto Larcher

S Bagga

S Chang

S Domingos

S Meir

BMC Plant Biology

PubMed

Background
Abscission is a highly coordinated developmental process by which plants control vegetative and reproductive organs load. Aiming at get new insights on flower abscission regulation, changes in the global transcriptome, metabolome and physiology were analyzed in ‘Thompson Seedless’ grapevine (Vitis vinifera L.) inflorescences, using gibberellic acid (GAc) spraying and shading as abscission stimuli, applied at bloom.
Results
Natural flower drop rates increased from 63.1 % in non-treated vines to 83 % and 99 % in response to GAc and shade treatments, respectively. Both treatments had a broad effect on inflorescences metabolism. Specific impacts from shade included photosynthesis inhibition, associated nutritional stress, carbon/nitrogen imbalance and cell division repression, whereas GAc spraying induced energetic metabolism simultaneously with induction of nucleotide biosynthesis and carbon metabolism, therefore, disclosing alternative mechanisms to regulate abscission. Regarding secondary metabolism, changes in flavonoid metabolism were the most represented metabolic pathways in the samples collected following GAc treatment while phenylpropanoid and stilbenoid related pathways were predominantly affected in the inflorescences by the shade treatment. However, both GAc and shade treated inflorescences revealed also shared pathways, that involved the regulation of putrescine catabolism, the repression of gibberellin biosynthesis, the induction of auxin biosynthesis and the activation of ethylene signaling pathways and antioxidant mechanisms, although often the quantitative changes occurred on specific transcripts and metabolites of the pathways.
Conclusions
Globally, the results suggest that chemical and environmental cues induced contrasting effects on inflorescence metabolism, triggering flower abscission by different mechanisms and pinpointing the participation of novel abscission regulators. Grapevine showed to be considered a valid model to study molecular pathways of flower abscission competence acquisition, noticeably responding to independent stimuli

Domingos, S.

Fino, J.

Cardoso, V.

Sánchez, C.

Ramalho, J. C.

Paulo, O. S.

Oliveira, C. M.

Goulao, L. F.

Larcher, Roberto

Archivio istituzionale della ricerca - Fondazione Edmund Mach

Shared and divergent pathways for flower abscission are triggered by gibberellic acid and carbon starvation in seedless Vitis vinifera L

Research ArticleBackground: Abscission is a highly coordinated developmental process by which plants control vegetative and
reproductive organs load. Aiming at get new insights on flower abscission regulation, changes in the global
transcriptome, metabolome and physiology were analyzed in ‘Thompson Seedless’ grapevine (Vitis vinifera L.)
inflorescences, using gibberellic acid (GAc) spraying and shading as abscission stimuli, applied at bloom.
Results: Natural flower drop rates increased from 63.1 % in non-treated vines to 83 % and 99 % in response
to GAc and shade treatments, respectively. Both treatments had a broad effect on inflorescences metabolism.
Specific impacts from shade included photosynthesis inhibition, associated nutritional stress, carbon/nitrogen
imbalance and cell division repression, whereas GAc spraying induced energetic metabolism simultaneously
with induction of nucleotide biosynthesis and carbon metabolism, therefore, disclosing alternative mechanisms
to regulate abscission. Regarding secondary metabolism, changes in flavonoid metabolism were the most
represented metabolic pathways in the samples collected following GAc treatment while phenylpropanoid
and stilbenoid related pathways were predominantly affected in the inflorescences by the shade treatment.
However, both GAc and shade treated inflorescences revealed also shared pathways, that involved the regulation of
putrescine catabolism, the repression of gibberellin biosynthesis, the induction of auxin biosynthesis and the activation
of ethylene signaling pathways and antioxidant mechanisms, although often the quantitative changes occurred on
specific transcripts and metabolites of the pathways.
Conclusions: Globally, the results suggest that chemical and environmental cues induced contrasting effects on
inflorescence metabolism, triggering flower abscission by different mechanisms and pinpointing the participation
of novel abscission regulators. Grapevine showed to be considered a valid model to study molecular pathways of
flower abscission competence acquisition, noticeably responding to independent stimuliinfo:eu-repo/semantics/publishedVersio

Domingos, Sara

Fino, Joana

Cardoso, Vânia

Sánchez, Claudia

Ramalho, José C.

Paulo, Octávio S.

Oliveira, Cristina M.

Goulao, Luis

UTL Repository

Shared and divergent pathways for flower abscission are trggered by gibberellic acid and carbon starvation in seedless Vitis

Universidade Técnica de Lisboa

Sara Domingos (3416096)

Joana Fino (3182070)

Vânia Cardoso (3416102)

Claudia Sánchez (3051630)

José Ramalho (359402)

Roberto Larcher (350249)

Octávio Paulo (3416099)

Cristina Oliveira (352628)

Luis Goulao (3416105)

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