Meiotic chromosome spreads in wild type, <i>spo11-1rec8</i>, <i>TDM1-P17</i> and <i>spo11-1rec8 TDM1-P17</i> plants.

Abstract

<p>(A to D) Meiosis in wild type. (A) Five bivalents align at metaphase I and (B) pairs of homologous chromosome are distributed into two nuclei at telophase I. (C) Five pairs of sister chromatids align on the two metaphase plates. (D) Four balanced nuclei are formed at telophase II. (E to H) Meiosis in <i>spo11-1rec8</i>. The first division resembles a mitotic division with (E) alignment of 10 pairs of chromatids on the metaphase plates and (F) segregation into two groups of 10 chromatids. (G) Single chromatids fail to align properly at metaphase II, resulting in (H) a variable number of unbalanced nuclei at telophase II. (I to J) Meiosis in wild type plants transformed with <i>TDM1-P17</i>. A single, meiosis I-like division is observed. (K to L) Meiosis in <i>spo11-1 rec8</i> plants transformed with <i>TDM1-P17</i>. A single, mitotic-like division is observed. Scale bar = 10μM.</p