Schedule of drug-escalating selection of GNA, CV-N, ConA and GRFT resistant HCV strains as a function of time.

Abstract

<p>HuH-7-RFP-NLS-IPS cells were infected in the presence of GNA (<b>A</b>), CV-N (<b>B</b>), ConA (<b>C</b>) or GRFT (<b>D</b>). Infected cells were subcultured every three to four days in the presence of the lectins. When 100% cells were infected (indicated by arrows), supernatants were recovered and used to infect naive cells in the presence of the lectins. The concentrations of each lectin were increased in a stepwise manner as indicated. Stars indicate the time points when the virus isolates were recovered and sequenced.</p