Influence of environmental factors on multixenobiotic resistance mechanism in earthworm Eisenia fetida, Savigny

Abstract

Mehanizam multiksenobiotičke otpornosti (MXR) dio je staničnog detoksifikacijskog mehanizma posredovan aktivnošću ABC transportnih proteina. Glavni predstavnici MXR mehanizma su P-glikoprotein (P-gp; ABCB1), multidrug resistance associated proteins (MRP1-5; ABCC1-5) i breast cancer resistance (BCRP; ABCG2) protein. Navedeni transmembranski prijenosnici prisutni su u svim organizmima i predstavljaju prvu liniju obrane organizma. Uz pomoć ATP-a izbacuju iz stanice endogene spojeve, ksenobiotike, uključujući antropogeno stvorene spojeve, spriječavajući na taj način potencijalno toksičan učinak u stanici (organizmu). Bisfenol A, zbog sve šire upotrebe u industriji polikarbonatne plastike postao je sveprisutno okolišno zagađivalo. U ovom radu po prvi puta je identificirana prisutnost MXR mehanizma i identificiran nukleotidni slijed gena koji kodira za P-glikoprotein u gujavici vrste E. fetida. Uz to, u navedenom modelnom organizmu istraživan je utjecaj bisfenola A i onečišćenih uzoraka snijega na MXR mehanizam.Multixenobiotic resistance mechanism (MXR) is cellular detoxification mechanism mediated by the activity of ATP-binding cassette (ABC) transporters. The main representatives of MXR mechanism are P-glycoprotein (P-gp; ABCB1), multidrug resistance associated proteins (MRP; ABCC1-5) and breast cancer resistance (BCRP; ABCG2) protein. These transmembrane efflux proteins are present in all organisms and represent a first line defence mechanism, by extrusion endogenous chemicals, xenobiotics, including man-made chemicals out of the cell (organism), thus preventing toxic effect. Bisphenol A is one of the highest volume chemicals produced worldwide because of their use in the manufacture of polycarbonate plastic. In this study was for the first time identified presence of MXR efflux activity, determined the partial sequence of P-gp/ABCB1 and defined impact of bisphenol A and polluted snow samples on detoxification mechanisms in earthworm Eisenia fetida