Excitons in the LH3 Complexes from Purple Bacteria

Abstract

The noncovalently bound and structurally identical bacteriochlorophyll <i>a</i> chromophores in the peripheral light-harvesting complexes LH2 (B800–850) and LH3 (B800–820) from photosynthetic purple bacteria ensure the variability of the exciton spectra in the near-infrared (820–850 nm) wavelength region. As a result, the spectroscopic properties of the antenna complexes, such as positions of the maxima in the exciton absorption spectra, give rise to very efficient excitation transfer toward the reaction center. In this work, we investigated the possible molecular origin of the excitonically coupled B820 bacteriochlorophylls in LH3 using femtosecond transient absorption spectroscopy, deconvolution of steady-state absorption spectra, and modeling of the electrostatic intermolecular interactions using a charge density coupling approach. Compared to LH2, the upper excitonic level is red-shifted from 755 to 790 nm and is associated with an approximate 2-fold decrease of B820 intrapigment coupling. The absorption properties of LH3 cannot be reproduced by only changing the B850 site energy but also require a different scaling factor to be used to calculate interpigment couplings and a change of histidine protonation state. Several protonation patterns for distinct amino acid groups are presented, giving values of 162–173 cm^–1 at 100 K for the intradimer resonance interaction in the B820 ring