Karyological analysis of freshwater crayfish Austropotamobius pallipes (Lereboullet, 1858) and Austropotamobius torrentium (Schrank, 1803)

Abstract

Provedeno je kariološko-citogenetičko istraživanje na europskim autohtonim vrstama rakova Austropotamobius pallipes i A. torrentium iz porodice Astacidae. Vrsta A. pallipes ima diploidan broj kromosoma 2n=168, a vrsta A. torrentium ima 2n=164. Formula kariotipa za vrstu A. pallipes je 148m+18sm+2a, a za vrstu A. torrentium je 148m+16sm. Pomoću metode pruganja flurorescencijskom bojom 4,6-diamidino-2-fenilindol (DAPI) zabilježeno je da većina kromosoma vrste A. pallipes i A. torrentium sadrži heterokromatinske blokove smještene centromerno i pericentromerno. Na nekim kromosomima vrste A. torrentium nisu primijećene centromerne pruge, a na nekim kromosomima obje vrste su primijećene interkalarne heterokromatinske pruge.The aim of this study was karyological investigation of the European native crayfish species Austropotamobius pallipes and A. torrentium. The karyotype of A. pallipes consists of 2n=168 chromosomes, while the karyotipe of A. torrentium consists of 2n=164. The karyotype formulas are 148m+18sm+2a and 148m+16sm for A. pallipes and A. torrentium, respectively. Fluorochrome staining with 4,6-diamino-2-phenylindole (DAPI) has revealed that the majority of chromosomes of both A. pallipes and A. torrentium are characterised by large heterochromatic blocks located in the centromeric and pericentromeric positions. Some of A. torrentium chromosomes didn’t show centromeric bands, while some of the chromosomes of both species revealed presence of intercalary positioned heterochromatc bands