Long-Term
Performance and Stability of Molecular Shotgun
Lipidomic Analysis of Human Plasma Samples
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Abstract
The stability of the lipid concentration
levels in shotgun lipidomics
analysis was tracked over a period of 3.5 years. Concentration levels
in several lipid classes, such as phospholipids, were determined in
human plasma lipid extracts. Impact of the following factors on the
analysis was investigated: sample amount, internal standard amount,
and sample dilution factor. Moreover, the reproducibility of lipid
profiles obtained in both polarity modes was evaluated. Total number
of samples analyzed was approximately 6800 and 7300 samples in negative
and positive ion modes, respectively, out of which 610 and 639 instrument
control samples were used in stability calculations. The assessed
shotgun lipidomics approach showed to be remarkably robust and reproducible,
requiring no batch corrections. Coefficients of variation (CVs) of
lipid mean concentration measured with optimized analytical parameters
were typically less than 15%. The high reproducibility indicated that
no lipid degradation occurred during the monitored time period