The role of HLA-DPB1 alleles on outcome of hematopoietic stem cell transplantation from unrelated donor

Abstract

U ovom radu analizirali smo podudarnost HLA među parovima primatelj-nesrodni davatelj u programu nesrodne transplantacije krvotvornih matičnih stanica (TKMS). Unutar ispitivane skupine odredili smo raspodjelu alela HLA-DPB1 među bolesnicima, njihovim nesrodnim davateljima i kontrolama, broj nepodudarnosti na lokusima HLA-A, -B, -C, -DRB1, -DQB1 i –DPB1 između primatelja i nesrodnog davatelja te ispitali povezanost dopuštenih i nedopuštenih nepodudarnosti HLA-DPB1 s ishodom TKMS. Skupina je obuhvatila 57 parova primatelj-davatelj kojima su određeni aleli HLA-A, -B, -C, -DRB1, -DQB1 i –DPB1 metodom lančane reakcije polimerazom i specifičnim početnicama ili probama za alele HLA (metode: PCR-SSP i PCR-SSO). Među bolesnicima najčešći alel bio je HLA-DPB1*04:01 (42,1%) što je u skladu s rezultatima za kontrolnu skupinu. Unutar cjelokupne skupine bolesnika nije otkrivena statistički značajna razlika u preživljavanju između bolesnika s 0, 1 ili 2 nepodudarnosti DPB1. Statistički značajno bolje preživljavanje bolesnika s 2 nepodudarnosti za alele DPB1 uočeno je samo u skupini bolesnika koji su imali davatelja 10/10. Nije uočena statistički značajna razlika u preživljavanju između bolesnika s dopuštenim i onih s nedopuštenim nepodudarnostima DPB1. Za donošenje konačnog zaključka o nepodudarnosti za alele HLA-DPB1 u ishodu TKMS neophodno je provesti veći broj istraživanja.In the present study the correlation between HLA mismatches among recipient-unrelated donor in hematopoietic stem cell transplantation (HSCT) were analyzed. Tested group comprised of 57 pairs. The distribution of DPB1 alleles in the group of patients, unrelated donors and healthy controls was compared. The next goal was to analyze the number of mismatches at HLA-A,-B,-C, -DRB1, -DQB1 and -DPB1 loci between recipient-donor pairs and their correlation on the outcome of HSCT. HLA alleles were tested by Polymerase Chain Reaction and specific primers or probes (PCR-SSP and PCR-SSO methods). Among all three tested groups DPB1*04:01 allele was the most frequent (42.1%). No statistically significant difference in survival after HSCT was observed between patients with 0, 1, or 2 mismatches at DPB1 locus. In the group of 10/10 matched pairs significantly better survival was observed for patients with 2 DPB1 mismatches. There was no statistically significant difference in survival between patients with permissive and non-permissive DPB1 mismatches. For the final conclusion about the necessity of matching for HLA-DPB1 alleles it is required to enlarge the number of studies