Fratila, R.M.

Grootendorst, D.J.

Haken, B. ten

Jose, J.

Leeuwen, A.G.J.M. van

Manohar, S.

Ruers, T.J.M.

Steenbergen, W.

Velders, A.H.

Visscher, M.

Grootendorst, Diederik

Velders, Aldrik

ten Haken, Bernard

van Leeuwen, Ton

Steenbergen, Wiendelt

Manohar, Srirang

Ruers, Theo J.M.

NARCIS 

Photoacoustic metastases detection using clinically approved SPIO dispersions

University of Twente Research Information

Top: Comparison in measured SPIO distribution between PA and 14 Tesla MR imagingSPIOs mainly distributed in the periphery of the nodal volume (white dotted line). Bottom: Node with and without SPIOs after paran embedding and H&E staining.  Measured amount of iron within each node (µg) and corresponding µa (mm-1) estimated using physical properties of the SPIO and Mie scattering simulations   Nodal metastasis detection using SPIOs for MR enhancement. K. Kimura, Breast Cancer, 2010   Clinically approved SPIO disperion used for pre-operative MR enhancementOutlook        PA detection of SPIOs creates possibilities for intra-operative nodal assessment in oncology       Evaluation of concept inside a metastatic animal model in progress      Rapid implementation into the clinic due to the availibility of clinically approved dispersions      Towards compact intra-operative imaging modality for resected lymph node assessment Tomographic photoacoustic setup   Curvilinear 32 element ultrasound detector array  Central frequency 6.25 MHz with 80% FBW  Elevation plane focus of 1 mm with axial resolution of 150 µm   Repetition rate: 10 Hz - Pulse duration: 10 ns      Projections:  20 (18 degree) - Energy: 20 mJ/cm2 at 720 nm      Averages: 100 - Slice acquistion time: 200 seconds      Patient prognosis and treatment based on accurate nodal staging   Pre-operative nodal MR staging for different malignancies can be improved        using Super Paramagnetic Iron Oxide (SPIO) nanoparticles      Uptake of SPIOs in healthy nodel tissue compared to absence        of uptake in malignant tissue  Photoacoustically imaging SPIO deposits within nodal tissue       could lead to a  fast intra- or pre-operative nodal staging technique           Introduction and Hypothesis      SPIO nanoparticles proven to improve nodal staging using MRI       Optical properties of SPIOs favorable for PA detection  Clinical approved SPIO dispersions commercially avalaible  Availibility facilitates rapid clinical applicability              Benefit of the approachNumber lymph node 1 2 3 4 5 6Iron amount (µg) 27 ± 2 51 ± 4 40 ± 3 49 ± 3 30 ± 2 11 ± 1Absorption coefficient µa (mm-1)0.14 ±0.010.27 ±0.020.21 ±0.020.26 ±0.020.15 ±0.010.06 ±0.01MRI Histology Anatomical layoutExperimental animal model water level32-element ultrasound detector array rotate around objectlymph  nodein bath of PBS agar  holderlaser lightin top-illumination      Evaluation in healthy rat model      Subcutaneous injection of 0.1 ml Endorem® in dorsal side hindleg      After 24 hours resection of the node      Photoacoustic and14 Tesla MR imaging of the resected lymph nodes      Additional verification of SPIO distribution and quantity using H&E       staining and Vibrating Sample Magnetometry      Nodal tissue shows no PA response without contrast injection  SPIO deposits within nodes possible to map using PA tomography       Distribution of SPIOs comparable with results of 14 T MRI and histology      SPIO deposition mostly in the periphery of the node          ResultsD. J. Grootendorst1 , J. Jose1, R.M. Fratila2 , M. Visscher2 , A.H. Velders3 , B. Ten Haken2 , T. G. van Leeuwen1,4 ,  W. Steenbergen1 ,  S. Manohar1 ,T. J. M. Ruers5 Photoacoustic metastases detection using clinically approved SPIO dispersions1Biomedical Photonic Imaging, 2Neuro IMaging group, 3Biomedical Chemistry, and 5Nanobiophysics GroupMIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands4Biomedical Engineering and Physics, University of Amsterdam, Academic Medical Center, Amsterdam, The NetherlandsResearch funded by the MIRA institute and AgentschapNL through the PRESMITT project IPD067771 in the theme IOP Photonic Devices

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http://doc.utwente.nl/83164/1/Grootendorst12photo.pdf

Photoacoustic metastases detection using clinically approved SPIO dispersions

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University of Twente Research Information