Biotinylation of Silicon and Nickel Surfaces and Detection
of Streptavidin as Biosensor
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Abstract
The availability of metal mesh device
sensors has been investigated
using surface-modified nickel mesh. Biotin was immobilized on the
sensor surfaces consisting of silicon and nickel via a thiol–ene
click reaction, known as the Michael addition reaction. Biotinylation
on the maleimidated surface was confirmed by X-ray photoelectron spectroscopy.
The binding of streptavidin
to the biotinylated surfaces was evaluated using a quartz crystal
microbalance and a metal mesh device sensor, with both techniques
providing similar binding constant value. The recognition ability
of the biotin immobilized using the thiol-maleimide method for streptavidin
was comparable to that of biotin immobilized via several other methods.
The adsorption of a biotin conjugate onto the streptavidin-immobilized
surface via the biotin–streptavidin–biotin sandwich
method was evaluated using a fluorescent microarray, with the results
demonstrating that the biological activity of the streptavidin remained