Development
of an Oligopeptide Functionalized Surface
Plasmon Resonance Biosensor for Online Detection of Glyphosate
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Abstract
We
report a surface plasmon resonance (SPR) biosensor for online
detection of glyphosate. The surface of the sensing element is decorated
with an oligopeptide, TPFDLRPSSDTR, which is identified by using phage
display library. This oligopeptide shows high binding specificity
for glyphosate (<i>K</i><sub>D</sub> = 8.6 μM), probably
because of the presence of R and D in the oligopeptide. To detect
glyphosate in buffer solution, an SPR gold sensor chip is modified
by using the oligopeptide with a surface density of 0.6 1/nm<sup>2</sup>. The sensitivity of this oligopeptide-functionalized SPR biosensor
is 1.02 RU/μM whereas the limit of detection (LOD) is 0.58 μM.
This oligopeptide functionalized SPR biosensor also shows good specificity
against other analytes such as glycine, thiacloprid, and imidacloprid