Thiol-Reactive Molecule with Dual-Emission-Enhancement
Property for Specific Prestaining of Cysteine Containing Proteins
in SDS-PAGE
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Abstract
1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene
(<b>2</b>) was synthesized and evaluated for specific fluorescent
prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The
molecule showed classic aggregation-induced emission (AIE) property
in protein labeling and its quantum efficiency was further enhanced
upon reacting with Cys. The parameters of reaction such as labeling
time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine
(TCEP) were examined to obtain the optimal labeling condition. In
addition to its specific labeling effect, molecule <b>2</b> also
showed its advantage over traditional self-quenching dyes through
labeling Cys containing BSA with different dye/Cys ratios