Extended Linear Response
for Bioanalytical Applications
Using Multiple Enzymes
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Abstract
We develop a framework for optimizing a novel approach
to extending
the linear range of bioanalytical systems and biosensors by utilizing
two enzymes with different kinetic responses to the input chemical
as their substrate. Data for the flow injection amperometric system
devised for detection of lysine based on the function of l-lysine-alpha-oxidase and lysine-2-monooxygenase are analyzed. Lysine
is a homotropic substrate for the latter enzyme. We elucidate the
mechanism for extending the linear response range and develop optimization
techniques for future applications of such systems