Nano Rolling-Circle Amplification
for Enhanced SERS Hot Spots in Protein Microarray Analysis
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Abstract
Although “hot spots” have been proved to
contribute to surface enhanced Raman scattering (SERS), less attention
was paid to increase the number of the “hot spot” to
directly enhance the Raman signals in bioanalytical systems. Here
we report a new strategy based on nano rolling-circle amplification
(nanoRCA) and nano hyperbranched rolling-circle amplification (nanoHRCA)
to increase “hot spot” groups for protein microarrays.
First, protein and ssDNA are coassembled on gold nanoparticles, making
the assembled probe have both binding ability and hybridization ability.
Second, the ssDNAs act as primers to initiate in situ RCA reaction
to produced long ssDNAs. Third, a large number of SERS probes are
loaded on the long ssDNA templetes, allowing thousands of SERS probes
involved in each biomolecular recognition event. The strategy offered
high-efficiency Raman enhancement and could detect less than 10 zeptomolar
protein molecules in protein microarray analysis