Measuring Uptake Dynamics
of Multiple Identifiable Carbon Nanotube Species via High-Speed Confocal
Raman Imaging of Live Cells
- Publication date
- Publisher
Abstract
Carbon nanotube uptake was measured via high-speed confocal
Raman imaging in live cells. Spatial and temporal tracking of two
cell-intrinsic and nine nanotube-derived Raman bands was conducted
simultaneously in RAW 264.7 macrophages. Movies resolved single (<i>n</i>, <i>m</i>) species, defects, and aggregation
states of nanotubes transiently as well as the cell position, denoted
by lipid and protein signals. This work portends the real-time molecular
imaging of live cells and tissues using Raman spectroscopy, affording
multiplexing and complete photostability